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Gregorian chant is the central Western tradition of plainchant: a monophonic, unaccompanied sacred song in Latin (and occasionally Greek) used in the Roman Catholic liturgy. It employs free rhythm guided by the prosody of the text rather than by strict meter, and is sung in unison by clerics or scholas. Its melodies are organized by the system of eight church modes, with characteristic finalis (final), tenor/reciting tones, and melodic formulas. Repertoires include the Proper and Ordinary of the Mass (e.g., Introit, Gradual, Alleluia, Offertory, Communion, Kyrie, Gloria, Credo, Sanctus, Agnus Dei) and the Divine Office (e.g., Antiphons, Responsories, Hymns, Psalms). Although legend credits Pope Gregory I, modern scholarship sees Gregorian chant as a Carolingian synthesis of Old Roman and Gallican chants, standardized across Frankish realms and later the broader Latin West.

Ambrosian chant is the liturgical plainchant of the Ambrosian Rite, practiced primarily in and around Milan, Italy. It is one of the few non-Gregorian Latin chant traditions to survive in continuous use, and is closely associated with the legacy of St. Ambrose (4th century). Like other plainchant, it is monophonic, unaccompanied, and sung in Latin, but it has its own melodic idioms, modal organization, and liturgical genres distinct from the Roman (Gregorian) tradition. Its rhythms are free and speech-like, designed to carry sacred texts clearly and reverently rather than follow metrical patterns. Ambrosian hymnody, often in iambic dimeter, is a hallmark, reflecting the formative influence of early Milanese practice and Eastern antiphonal singing.